2018-Sustainable Industrial Processing Summit
SIPS2018 Volume 2. Amatore Intl. Symp. / on Electrochemistry for Sustainable Development
| Editors: | F. Kongoli, H. Inufasa, M. G. Boutelle , R. Compton, J.-M. Dubois, F. Murad |
| Publisher: | Flogen Star OUTREACH |
| Publication Year: | 2018 |
| Pages: | 216 pages |
| ISBN: | 978-1-987820-84-3 |
| ISSN: | 2291-1227 (Metals and Materials Processing in a Clean Environment Series) |
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Highly Sensitive Electrochemical Bioanalysis of Unicellular Samples based on Au@Pt Nanaostructures Modified Diamond Microelectrode Arrays
Xuemei Wang1; Hui Jiang1; Alberto Pasquarelli2; Chunmei Li1; Hang Zhang1; Jun Ruan1;1SOUTHEAST UNIVERSITY, Nanjing, China; 2ULM UNIVERSITY, Ulm, Germany;
Type of Paper: Regular
Id Paper: 229
Topic: 47
Abstract:
In this work, aiming at the construction of a sensitive, disposable system for the bioanalysis of unicellular samples, we report a single cell detection electrochemical (SCDE) platform based on Au@Pt nanostructures modified boron-doped nanocrystalline diamond microelectrode arrays (BNCD-MEAs) with 16 channels. The SCDE platform could be readily adapted for a highly sensitive detection of H<sub>2</sub>O<sub>2</sub> released from stimulated single cell by ascorbic acid (AA), which could be utilized to distinguish cancer unicellular and normal unicellular ones. The Au@Pt nanostructures have good electrocatalytic activity for the H<sub>2</sub>O<sub>2</sub> detection and were modified in the BNCD-MEAs by Pt NPs which were electrodeposited onto the BNCD-MEAs through an electrodeposition approach. As a result, this SCDE platform exhibited a wide linear range with a low detection for H<sub>2</sub>O<sub>2</sub> determination. Furthermore, it also displayed satisfactory selectivity, excellent stability, and good reproducibility. The developed method opens a new pathway to clinical bioassay.
Keywords:
Bioelectrochemistry;References:
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