2019-Sustainable Industrial Processing Summit
SIPS2019 Volume 11: New and Advanced Materials, Technologies, and Manufacturing
| Editors: | F. Kongoli, F. Marquis, N. Chikhradze, T. Prikhna |
| Publisher: | Flogen Star OUTREACH |
| Publication Year: | 2019 |
| Pages: | 174 pages |
| ISBN: | 978-1-989820-10-0 |
| ISSN: | 2291-1227 (Metals and Materials Processing in a Clean Environment Series) |
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Resonance Raman investigation of the Reaction of Cytochrome c Oxidase NO2
Charalampos Tselios1; Constantinos Varotsis2;1CYPRUS UNIVERSITY OF TECHNOLOGY, Limassol, Cyprus; 2CYPRUS UNIVERSITY OF TECHNOLOGY, limassol, Cyprus;
Type of Paper: Regular
Id Paper: 238
Topic: 43
Abstract:
The active center of cytochrome c oxidases is a binuclear center containing a heme and a copper atom. Both metal centers (heme Fe and CuB) may be involved in NO2 coordination and reduction since bona fide reductases are either heme-containing or copper- containing enzymes.1,2,3
We will present Raman experiments of the reaction of Cytochrome c oxidase with NO2 to determine the metal where NO2 coordinates the redox active center that provides the required electron for NO2- reduction. The structural properties of the intermediates that are formed upon NO2- reduction by CcO are described.
Raman data was collected by a confocal LabRAM (HORIBA JobinYvon, Kyoto, Japan) equipped with a CCD detector and a grating of 1800 grooves/mm. The 514.1 nm excitation beam was provided by a Coherent Sapphire laser.
Keywords:
Biomaterials;References:
1) E Pinakoulaki, C Varotsis, 2008 ,Journal of inorganic biochemistry 102 (5-6), 1277-12872) E Pinakoulaki, C Varotsis, 2003 , Biochemistry 42 (50), 14856-14861
3) E Pinakoulaki, S Stavrakis, A Urbani, C Varotsis, 2002 ,Journal of the American Chemical Society 124 (32), 9378-9379