| Developing New Tools for Understanding the Processes of Oxidative Stress in the Brain Yang Tian1; 1SCHOOL OF CHEMISTRY AND MOLECULAR ENGINEERING, EAST CHINA NORMAL UNIVERSITY, Shanghai, China; PAPER: 536/Oxidative/Keynote (Oral) SCHEDULED: 12:45/Wed. 30 Nov. 2022/Ballroom A ABSTRACT: Our group focuses on developing new devices and analytical methods for tracking of chemical and electrical signals simultaneously in the brain of free-moving animals. Systematic and in-depth research has been carried out on breaking the key bottleneck of real-time monitoring and quantification of from reactive oxygen species and oxidative stress related species in physiological and pathological processes with high selectivity, high accuracy, long-term stability, and designing new devices for brain imaging with high temporal resolution.[1-2] Firstly, the synergy strategy of molecular recognition and electrochemical recognition was proposed for achieving highly selective determination, and new approaches based on rational design of specific molecules with built-in calibration were established for accurate quantification in living brains.[3] Secondly, to challenge the real-time determination in the complicated brain, Au-S, Au-Se and Au-C≡C bonds were systematically investigated. The Au-C≡C bond showed the highest stability under thiol-rich biological conditions and the best electrochemical performance compared to the others. Furthermore, a more reliable sensing platform with long-term stability and anti-biofouling was constructed through rational integrating highly stable graphene-layered molecular interface, remarkably elongating the time dimension for real-time tracking of the dynamic changes in free-moving animals up from several hours to a record-long 60 days.[4] Thirdly, a SERS optophysiological probe was created for real-time mapping and recording of chemical and electrical signals without cross-talk in the live brain. Using this powerful tool, three new routes that causes Cu+ and Cu2+ change were discovered during ischemia: export from neurons; release from digested copper-containing proteins; conversion from Cu+ to Cu2+. Moreover, it was the first time that a Raman fibre photometry was built up for real-time tracking and simultaneous quantitation of multiple molecules in mitochondrial across the brain of free-moving animals. Meanwhile, a highly selective non-metallic Raman probe was created through triple-recognition strategies of chemical reaction, charge transfer, and characteristic fingerprint peaks, for monitoring and quantifying of local mitochondrial O2•-, Ca2+ and pH in six brain regions upon hypoxia. It was discovered that hypoxia-induced mitochondrial O2•- burst was regulated by ASIC1a, leading to mitochondrial Ca2+ overload and acidification.[5-7] References: 1. Y. Liu, Z. Liu, Y. Tian. Acc. Chem. Res. 2022, DOI: 10.1021/acs.accounts.2c00333. 2. Y. Mei, Q. Zhang, Q. Gu, Z. Liu, X. He, Yang Tian. J. Am. Chem. Soc. 144, 2351-2359 (2022). 3. Zhou Wu, Mengmeng Liu, Zhichao Liu, Yang Tian. J. Am. Chem. Soc. 142, 16, 7532-7541 (2020). 4. Y. Liu, Z. Liu, F. Zhao, Y. Tian. Angew. Chem. Int. Ed. 60, 14429-14437 (2021). 5. Z. Liu, Z. Zhang, Y. Liu, Y. Mei, E. Feng, Y. Liu, T. Zheng, J. Chen, S. Zhang, Y. Tian. Angew. Chem. Int. Ed. 61, e202111630 (2022). 6. W. Wang, F. Zhao, M. Li, C. Zhang, Y. Shao, Y. Tian. Angew. Chem. Int. Ed. 58, 5256-5260 (2019). 7. E. Feng, T. Zheng, J. Chen, Y. Tian. Sci. Adv. 4, eaau3494 (2018). |
