2023-Sustainable Industrial Processing Summit
Guerrant Intl. Symp /Covid-19 and Infectious Diseases
| Editors: | F. Kongoli, F. Murad, W. Petri, H. Inufusa, S. Heysell, J. Yedoyan |
| Publisher: | Flogen Star OUTREACH |
| Publication Year: | 2023 |
| Pages: | 92 pages |
| ISBN: | 978-1-989820-80-3 (CD) |
| ISSN: | 2291-1227 (Metals and Materials Processing in a Clean Environment Series) |
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QUANTITATIVE PCR OF STRING-TEST COLLECTED GASTRIC MATERIAL: A FEASIBLE APPROACH TO DETECT HELICOBACTER PYLORI AND ITS RESISTANCE AGAINST CLARITHROMYCIN AND LEVOFLOXACIN FOR SUSCEPTIBILITY-GUIDED THERAPY
Barry James Marshall1; Xinyuan Han2; Xiqiu Yu3; Xiaojuan Gao3; Xiangyu Wang4; Alfred Chin Yen Tay5; Xiaolan Wei3; Bing Lai3; Xiuming Zhang3; Eng Guan Chua1;1UNIVERSITY OF WESTERN AUSTRALIA, Crawley, Australia; 2ANHUI UNIVERSITY OF SCIENCE AND TECHNOLOGY, Anhui, China; 3THE THIRD AFFILIATED HOSPITAL OF SHENZHEN UNIVERSITY, Shenzhen, China; 4THE FIRST AFFILIATED HOSPITAL OF SHENZHEN UNIVERSITY, Shenzhen, China; 5UNIVERSITY OF WESTERN AUSTRALIA, Crawley, China;
Type of Paper: Regular
Id Paper: 119
Topic: 68
Abstract:
Background
As the reduced eradication rate of Helicobacter pylori (H. pylori), we introduced string-test and quantitative PCR (qPCR) for susceptibility-guided therapy innovatively. The practicality of the string test was evaluated.
Methods
It was an open-label, non-randomized, parallel, single-center study, in which subjects tested by 13C- urea breath test (UBT) and string-qPCR were enrolled. Based on the results of string-qPCR, we calculated clarithromycin and levofloxacin resistance rates and gave 13C-UBT positive patients 14 days susceptibility-guided bismuth quadruple therapy. In the empirical therapy group, we retrospectively analyzed the treatment results of 13C-UBT positive patients also treated with bismuth quadruple at Shenzhen Luohu People's Hospital from January 2021 to May 2022. The eradication rate was compared between susceptibility-guided therapy and empirical therapy groups.
Results
The diagnosis of H. pylori infection using the string-qPCR had an overall concordance rate of 95.9% with the 13C-UBT results. Based on the results of string-qPCR, the clarithromycin and levofloxacin resistance rates were 26.1% and 31.8%, respectively. The patients who were given 14 days susceptibility-guided bismuth-based quadruple therapy achieved a high H. pylori eradication rate of 91.8%. Retrospective analysis of patient treatment data from January 2021 to May 2022 available in the hospital database revealed an overall success rate of 82.3% for those who received empirical bismuth-based quadruple therapies, which is marginally significantly lower than that of the string-qPCR susceptibility-guided group (p = 0.084).
Conclusion
The high treatment success rate of 91.8% indicates that the string-qPCR test is a valuable and feasible approach for clinical practice to help improve H. pylori treatment success rate.