2025 - Sustainable Industrial Processing Summit
SIPS2025 Volume 14. Intl. Symp on Multiscale, Modelling, Nanotechnology and Modelling Materials
| Editors: | F. Kongoli, D. Bammann, R. Das, J.B. Jordon, R. Prabhu, A. Rajendran, P. Trovalusci, M. de Campos |
| Publisher: | Flogen Star OUTREACH |
| Publication Year: | 2025 |
| Pages: | 214 pages |
| ISBN: | 978-1-998384-64-8 (CD) |
| ISSN: | 2291-1227 (Metals and Materials Processing in a Clean Environment Series) |
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PROTECTIVE EFFECTS OF GELATIN-ENCAPSULATED QUERCETIN AGAINST OXIDATIVE CELL DAMAGE
Alla Potapovich1; Tatyana Kostyuk1; Tatsiana Shutova2; Vladimir Kostyuk1;1BYELORUSSIAN STATE UNIVERSITY, Minsk, Belarus; 2INSTITUTE OF CHEMISTRY OF NEW MATERIALS, Minsk, Belarus;
Type of Paper: Regular
Id Paper: 210
Topic: 69
Abstract:
Several studies have demonstrated the antioxidant and anti-inflammatory effects of plant polyphenols (PP) [1, 2]. However, despite their biological potential, the clinical application of PP is limited primarily by their poor water solubility, which results in low bioavailability when administered orally. Micro- and nanoparticles loaded with plant polyphenols have shown high pharmacological activity, making the development of such delivery systems and the investigation of their biological effects highly relevant [3]. The objective of this study was to compare the protective effects of native and nanostructured quercetin on the initiation of oxidative stress in human keratinocytes exposed to tert-butyl hydroperoxide (tBHP). Quercetin was encapsulated within gelatin-based microcontainers, forming nanoparticles with diameters ranging from 160 to 190 nm. Two formulations were used: uncoated gelatin nanoparticles (nano 1) and gelatin nanoparticles coated with a shell composed of dextran sulfate and a chitosan-dextran copolymer (nano 2). Cell viability was assessed using PrestoBlue™ reagent. Keratinocyte damage was evaluated via lactate dehydrogenase (LDH) release. Apoptotic and necrotic cells were identified through flow cytometry using an Annexin V-FITC/PI staining kit. DNA damage was analyzed using the comet assay. The results demonstrate that gelatin nanoparticles effectively encapsulate quercetin, and the nanostructured form enables its application in aqueous suspensions without compromising its antioxidant, gene-protective, and cytoprotective effects under conditions of cellular oxidative stress. Both free and nanoparticle-loaded quercetin significantly protected keratinocytes from oxidative DNA damage and apoptosis induced by tBHP. These findings suggest that gelatin nanoparticles are effective carriers for quercetin, exhibiting high efficiency in its release.
Conclusion: The use of gelatin nanoparticles represents a promising strategy for enhancing the bioavailability and therapeutic efficacy of phytochemicals.