Biomacromolecules such as DNA, proteins, and polysaccharides possess unique helical structures, which are closely related to various biological activities involving recognition, catalysis, replication, genetic information storage, etc.^[1-3] To date, many artificial foldamers have been designed because they can act as the ideal systems to simulate the structures and functions of biomacromolecules.^[4] Herein, we have synthesized a water-soluble poly(m-phenylene ethynylene)-based foldamer bearing L-alanine sodium pendants, whose solvent-driven helix inversion can be visualized with the aid of a tetraphenylethene-functionalized hemicyanine dye based on the restriction of intramolecular motions (RIM) and intramolecular charge transfer (ICT) mechanisms. In addition, another foldamer bearing L-alanine hexyl ester pendants is synthesized, showing a weak circularly polarized luminescence (CPL) signal in solution, but has a significant blue-colored negative CPL signal with a dissymmetry factor (g_lum) of -0.01 in film.  A benzothiadiazole dye-based achiral fluorescent film is prepared. Then, the two films are arranged side by side. When the foldamer film is close to the excitation light, a yellow-colored negative CPL signal with a glum value of -0.005 is observed due to the circularly polarized light excitation. Interestingly, the change of the position of the foldamer film leads to an opposite CPL signal with a glum value of +0.028 because the left-handed CPL is absorbed by the foldamer film. The present investigation is crucial in deepening our understanding of the foldamer conformations and promoting the development of novel CPL materials.