Research on Heat Shock Proteins (HSPs) is widely conducted, and their name suggests that they are proteins primarily responsive to heat stimuli. However, some HSPs respond to oxidative stress (OS) and inflammation.

HSP70, with a molecular weight of 70 kDa, is conserved in nearly all biological species and is the most well-characterized HSP in response to oxidative stress, with numerous reports available. HSP70 not only responds to OS but also has been reported to increase oxidative stress when inhibited. HSP90 is another important HSP, which is constantly expressed and maintains the structure of the NOX enzyme family that produces reactive oxygen species (ROS), thereby promoting ROS production.HSP90 inhibitors are being studied as anticancer agents. HPS27 is strongly induced by heat, OS, and drugs, and its levels significantly increase in cancer and OS responses. Since opportunities for OS to rise and inflammation to occur are more common in humans than prolonged exposure to saunas or hot baths, it is considered that OS triggers HSP responses in the human body. Following the development of Twendee X (TwX), safe antioxidant therapy for humans has become possible.TwX is a supplement composed of eight vitamins, amino acids, CoQ10, and other ingredients, and its efficacy has been confirmed in human and animal experiments for the following conditions:Dementia, cancer, hypertension, atherosclerosis, asthma, atopic dermatitis, hay fever, sinusitis, systemic sclerosis, and other allergic diseases; hearing loss, tinnitus, vocal cord fatigue, and other otolaryngological disorders; ALS, Parkinson's disease, post-stroke sequelae, and other neurological disorders; pancreatitis, ulcerative colitis, hepatitis, and other inflammatory diseases.TwX is an antioxidant formulation that can be safely used in both clinical and basic research, and it is expanding significantly in the field of antioxidant therapy, which has previously been challenging. In terms of HSP research, it is anticipated that combining OS stimulation with TwX will promote HSP research.