ORAL

SESSION: AdvancedMaterialsWedPM-R6	Marquis International Symposium on New and Advanced Materials and Technologies for Energy, Environment and Sustainable Development(3rd Intl Symp. on New and Advanced Materials and Technologies for Energy, Environment and Sustainable Development)
Wed Oct, 25 2017	Room: Condesa IA
Session Chairs: Hassane Oudadesse; Thorsten Kluener; Session Monitor: TBA

14:30: [AdvancedMaterialsWedPM05]
Synthetic Bone Biomaterial: Physicochemical and Biological Investigations
Hassane Oudadesse¹ ; S Mosbahi² ; S Jebahi³ ; A. Lucas Girot² ; H. Elfeki³ ; A. Elfeki³ ; G. Cathelineau² ; ¹University of rennes 1, Rennes, France; ²University of Rennes 1, Rennes, France; ³University of Sfax, Sfax, Tunisia;
Paper Id: 190 [Abstract]

Synthetic biomaterials such as calcium phosphate, calcium carbonate, geopolymers and bioactive glasses offer several opportunities for applications in orthopedic or jawbone surgery. Physicochemical and biological properties such as crystalline structure, mechanical properties, biocompatibility and kinetic of ossification are necessary for the success of biomaterials. In this work calcium carbonate in the aragonite form and bioactive glasses have been studied. Their association with antibiotic, biopolymers or bisphosphonate for the treatment of bone pathologies were performed. Calcium carbonate was synthesized at low temperatures. Obtained powder was compacted, mixed with porogens to create porosity (44% in this study) and implanted in the femoral site of ovine. A bioactive glass was elaborated by melting process and implanted in the tibia plates of rabbits. Several physicochemical techniques X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Neutron Activation Analysis (NAA) and Protons Induced X-rays Emission (PIXE) were employed to evaluate the in vitro and the in vivo behavior of these biomaterials. Histological studies were performed to analyze the eventual inflammations in the implanted area and to highlight the effects of introduced organic molecules in the matrix of biomaterials on the osteoporosis phenomenon. Obtained results show that mineral composition of these two biomaterials undergoes some transformations versus time of implantation in the femoral site. Concentrations of atomic elements such as Ca, P, Sr and Zn present spectacular variations versus time. Few weeks for bioactive glass and few months of CaCO3 after implantation, mineral composition of the initial implants is close to the mineral composition of the mature bone. Cartographies were performed on the surfaces of the biomaterials, on the interface bonebiomaterial and on the bone. Obtained results show a good bioconsolidation of these two biomaterials. Treatment of osteoporosis was observed when a specific bisphosphonate is present in the biomaterial matrix.

SESSION: AdvancedMaterialsWedAM-R6	Marquis International Symposium on New and Advanced Materials and Technologies for Energy, Environment and Sustainable Development(3rd Intl Symp. on New and Advanced Materials and Technologies for Energy, Environment and Sustainable Development)
Wed Oct, 25 2017	Room: Condesa IA
Session Chairs: Qizhen Li; Kunichi Miyazawa; Session Monitor: TBA

15:30: [AdvancedMaterialsWedAM07]
Hybrid Bio-Composite Glass-Chitosan Used for Applications in Biomedical Field
Hassane Oudadesse¹ ; S Mosbahi² ; S Jebahi³ ; H. Elfeki³ ; A. Elfeki³ ; H. Keskes³ ; ¹University of rennes 1, Rennes, France; ²University of Rennes 1, Rennes, France; ³University of Sfax, Sfax, Tunisia;
Paper Id: 191 [Abstract]

The hybrid composite consisting on the association of bioactive glass and chitosan biopolymer was elaborated by using an original process based on freeze-dried process and lyophilisation principle. This hybrid biocomposite offers several applications and advantages in dental,maxilo-facial and orthopedic surgery. Using this kind of biomaterial could enhance a direct bond to the living bone through the development of the surface layer of carbonated apatite and serves for therapeutic treatment such as osteoporosis phenomenon. The bioactive glass was synthesized in the SiO2 (46% wt.) - CaO (24% wt.) - Na2O (24% wt.) and P2O5 (6%wt.) system. The amount of introduced chitosan was of 17% wt. Chitosan was mixed with glass powder following several steps. Hybrid bio-composite was then implanted in the femoral site of ovarioctomized rates. Sampling has been carried out after a different period. Biological and physicochemical characterizations such as XRD, SEM, FTIR, MAS-NMR, mechanical characteristics and other techniques have employed to highlight this bio- composite behavior after in vivo assays. Effects of chitosan on the osteoporosis created following the ovarioctomization have been studied. Obtained results show good biocompatibility of bio-composite. The biological apatite is deposed since the first month after implanted as showed by SEM. It crystallizes in hexagonal system, which corresponds to the crystallographic structure of mature bone. 13C solid-state MAS NMR investigated the organic moiety of the bone Extra Cellular Matrix (ECM). Obtained spectra of native femoral condyle bone, show the typical spectral signature of collagen. 29Si and 31P were also investigated. The kinetic of ossification revealed that at 3 months of implantation, the hybrid bio-composite was completely transformed into bone. Osteoinduction phenomenon and antiosteoporotic effects of chitosan were highlighted in the ovariectomised rates by using histological investigations.